Tooth decay is an infectious disease caused by cariogenic bacteria. Caries is affected by the entire microbial community of the oral cavity, but some bacteria have a major impact. These are mainly two bacteria of the genus Strepotococcus: S. sobrinus and S. mutans, which adhere easily to the tooth surface and thrive in dental plaque. These two bacteria are among the so-called primary causes of tooth decay; they metabolize saccharides in dental plaque to acids that disrupt (demineralize) tooth enamel, resulting in tooth decay. The VariOr® Caries test is used to determine the risk of tooth decay in children. The test works on the principle of DNA detection of the above-mentioned bacteria.
Dental diseases in young children have a number of specifics - young children often do not cooperate in performing regular oral hygiene, as well as in examinations and necessary interventions at the dentist, but at the same time their teeth are sensitive to tooth decay. Prevention is therefore very important for young children. It consists mainly in observing the principles of oral hygiene, but also in preventing the transmission of caries-forming bacteria from the parents to the toddler at the time when the child's first teeth are cut. It has been shown that S. sobrinus and S. mutans infections in preschool children correlate with the number of existing caries, but especially with the future development of new caries. However, if the infection with these bacteria does not occur until approximately 7 years of age, when the oral microflora forms, the child is more resistant to infection by these microorganisms at a later age and has fewer problems with teeth for life (lower tooth decay). It has been shown that the highest risk of dental caries is in children infected with both pathogens at the same time, lower in children infected with S. sobrinus only, even lower in children infected with S. mutans and the lowest risk in children in whom neither pathogen was detected.
As striking as it is, the most common carrier of cariogenic bacteria is the mother or another member of the family. The infection occurs at the time of cutting the first teeth (baby teeth), when the mother licks the pacifier in an attempt to clean it, or tastes the food served to the child with the same spoon, which the child then being fed. Therefore, we recommend performing the test on both the child and the mother at the same time to determine the degree of risk of infection of the child. In addition, the number of bacteria in the oral cavity can be reduced by appropriate preventive interventions and thus reduce the possibility of mother-to-child transmission. You can take the sample yourself or at your dentist.
Samples are taken with a sterile cotton swab, preferably in the morning before brushing teeth. A special sampling kit can be ordered from the contacts listed below. A swab is applied to the surface of the entire tooth with a stick, similar to brushing teeth. (Use a second stick to smear the surface of the child's cut teeth.) Wet sticks must be left for at least 30 minutes to dry. For successful analysis, the rods must be perfectly dry! The stick, each separately, is placed back in the case marked with the name and surname of the person taken (or in a clean plastic bag, which is sealed and marked with the name and surname of the person taken). The samples are sent to the laboratory together with the application form. This is a paid service. The examination of one sample costs CZK 480 including VAT. We recommend making a payment by bank transfer. The service can also be paid for by cash on delivery, but in this case, we charge an additional fee of CZK 100. If the customer is a dental office, it is possible to make a payment on the invoice.
The test result determines the tendency to form tooth decay. In the case of testing mothers and children, it determines the level of risk of transmission of bacteria to the child, or confirms or refutes the presence of bacteria in the child's oral cavity. Based on the results, the dentist decides on a suitable hygiene plan and further treatment (re-mineralization, antimicrobials, frequency of check-ups, etc.).
Laboratory of Molecular Biology
Hůry 149 | Rudolfov | 373 71
Phone: +420 608 636 119, +420 602 156 577
Bank account number / code: 184419542/0300
Telefon: +420 608 636 119, +420 602 156 477
Lapirattanakul J., Nakano K., Nomura R., Hamada S., Nakagawa I., Ooshima T.(2008): Demonstration of mother-to-child transmission of Streptococcus mutans using multilocus sequence typing. Caries Res. 42(6):466-74.
Okada M., Soda Y., Hayashi F., Doi T, Suzuki J., Miura K., Kozai K. (2005) Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children. Med. Microbiol.: 54, 661–665.
Thorild I., Lindau B., Twetman S. (2006): Caries in 4-year-old children after maternal chewing of gums containing combinations of xylitol, sorbitol, chlorhexidine and fluoride. Eur Arch Paediatr Dent. 7(4): 241-5.
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